Helena Tattermuschová (born June 28, 1933) is a Czech lyric coloratura soprano, known chiefly for her character roles in the operas of Mozart and Janáček. Tattermuschová was born in Prague and studied vocal performance with Vlasta Linhartová at the Academy of Music in Prague. Upon completion of her studies, she was engaged by the Ostrava Opera where she made her stage debut as Musetta in 1955. By 1956, she was a member of the Prague National Theater opera company where she continued to sing well into the 1980s. She possessed a high lyric soprano voice that tended to cast her into either “daughter” roles or youthful trouser roles for many years into her career.